Defining Evolutionary Conservation Units in the Macedonian Crested Newt, Triturus macedonicus (Amphibia; Salamandridae), in a Biodiversity Hotspot

In this study, we used genetic approaches to assess the conservation status of a protected amphibian species, Macedonian crested newt, Triturus macedonicus, in Northern Pindos National Park (Epirus, Greece). Mitochondrial DNA sequences and multilocus genotypes individuals from 38 breeding sites were infer their phylogenetic position detect measure variation patterns, population structure, levels gene flow. The examined fell within two major clades newt phylogeny, being geographically separated by Aoos River valley Vikos Gorge. Both groups constitute separate pools, bearing private haplotypes alleles, found be highly differentiated both mitochondrial microsatellite markers. Thus, they meet all criteria needed characterized as evolutionary significant units (ESUs) that deserve status. Within each ESU, particularly high inter-population differentiation low migration rates imply lack connectivity between local isolation favorable habitats. Our results can inform future programs actions towards management area.